{"id":252413,"date":"2024-04-13T13:36:25","date_gmt":"2024-04-13T13:36:25","guid":{"rendered":"https:\/\/namso-gen.co\/blog\/?p=252413"},"modified":"2024-04-13T13:36:25","modified_gmt":"2024-04-13T13:36:25","slug":"what-is-a-good-blast-e-value","status":"publish","type":"post","link":"https:\/\/namso-gen.co\/blog\/what-is-a-good-blast-e-value\/","title":{"rendered":"What is a good blast E value?"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_62 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title \" >Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/namso-gen.co\/blog\/what-is-a-good-blast-e-value\/#What_is_a_good_blast_E_value\" title=\"What is a good blast E value?\">What is a good blast E value?<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/namso-gen.co\/blog\/what-is-a-good-blast-e-value\/#FAQs\" title=\"FAQs\">FAQs<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/namso-gen.co\/blog\/what-is-a-good-blast-e-value\/#1_How_is_the_blast_E_value_calculated\" title=\"1. How is the blast E value calculated?\">1. How is the blast E value calculated?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/namso-gen.co\/blog\/what-is-a-good-blast-e-value\/#2_Is_a_blast_E_value_of_0_possible\" title=\"2. Is a blast E value of 0 possible?\">2. Is a blast E value of 0 possible?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/namso-gen.co\/blog\/what-is-a-good-blast-e-value\/#3_What_does_an_E_value_of_1_mean\" title=\"3. What does an E value of 1 mean?\">3. What does an E value of 1 mean?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/namso-gen.co\/blog\/what-is-a-good-blast-e-value\/#4_What_is_considered_a_significant_blast_E_value\" title=\"4. What is considered a significant blast E value?\">4. What is considered a significant blast E value?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/namso-gen.co\/blog\/what-is-a-good-blast-e-value\/#5_Can_different_searches_have_the_same_E_value\" title=\"5. Can different searches have the same E value?\">5. Can different searches have the same E value?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/namso-gen.co\/blog\/what-is-a-good-blast-e-value\/#6_Is_a_lower_blast_E_value_always_better\" title=\"6. Is a lower blast E value always better?\">6. Is a lower blast E value always better?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/namso-gen.co\/blog\/what-is-a-good-blast-e-value\/#7_Can_the_blast_E_value_be_negative\" title=\"7. Can the blast E value be negative?\">7. Can the blast E value be negative?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-10\" href=\"https:\/\/namso-gen.co\/blog\/what-is-a-good-blast-e-value\/#8_Is_the_blast_E_value_affected_by_the_size_of_the_database\" title=\"8. Is the blast E value affected by the size of the database?\">8. Is the blast E value affected by the size of the database?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-11\" href=\"https:\/\/namso-gen.co\/blog\/what-is-a-good-blast-e-value\/#9_How_important_is_the_blast_E_value_in_sequence_analysis\" title=\"9. How important is the blast E value in sequence analysis?\">9. How important is the blast E value in sequence analysis?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-12\" href=\"https:\/\/namso-gen.co\/blog\/what-is-a-good-blast-e-value\/#10_Why_is_a_small_E_value_desirable\" title=\"10. Why is a small E value desirable?\">10. Why is a small E value desirable?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-13\" href=\"https:\/\/namso-gen.co\/blog\/what-is-a-good-blast-e-value\/#11_Can_the_blast_E_value_be_used_to_compare_different_queries\" title=\"11. Can the blast E value be used to compare different queries?\">11. Can the blast E value be used to compare different queries?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-14\" href=\"https:\/\/namso-gen.co\/blog\/what-is-a-good-blast-e-value\/#12_Are_there_any_limitations_to_the_blast_E_value\" title=\"12. Are there any limitations to the blast E value?\">12. Are there any limitations to the blast E value?<\/a><\/li><\/ul><\/li><\/ul><\/nav><\/div>\n<h2><span class=\"ez-toc-section\" id=\"What_is_a_good_blast_E_value\"><\/span>What is a good blast E value?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>The E value is a numerical parameter that measures the significance of sequence alignments performed using the Basic Local Alignment Search Tool (BLAST). It represents the expected number of alignments occurring by chance when searching a database. A lower E value suggests a higher level of significance, indicating a better match between query and subject sequences. So, the answer to the question &#8220;What is a good blast E value?&#8221; is simple: a good E value is a small one.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"FAQs\"><\/span>FAQs<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<h3><span class=\"ez-toc-section\" id=\"1_How_is_the_blast_E_value_calculated\"><\/span>1. How is the blast E value calculated?<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>\nThe blast E value is calculated by taking into account the database size, query sequence length, and the score of the alignment. It estimates the probability of observing an alignment with similar scores by chance.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"2_Is_a_blast_E_value_of_0_possible\"><\/span>2. Is a blast E value of 0 possible?<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>\nA blast E value of 0 is not possible, as it would indicate a perfect match with no chance of obtaining the observed alignment by chance.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"3_What_does_an_E_value_of_1_mean\"><\/span>3. What does an E value of 1 mean?<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>\nAn E value of 1 represents the expected number of alignments with similar scores that one might find in a randomly generated database of equal size.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"4_What_is_considered_a_significant_blast_E_value\"><\/span>4. What is considered a significant blast E value?<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>\nA significant E value is usually below 0.01, indicating a very low chance of getting the observed alignment by random chance.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"5_Can_different_searches_have_the_same_E_value\"><\/span>5. Can different searches have the same E value?<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>\nYes, different searches can have the same E value, suggesting the same level of significance. However, the alignment scores and sequence lengths still vary.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"6_Is_a_lower_blast_E_value_always_better\"><\/span>6. Is a lower blast E value always better?<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>\nYes, a lower E value indicates a higher level of significance, suggesting a better match between the query and subject sequences.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"7_Can_the_blast_E_value_be_negative\"><\/span>7. Can the blast E value be negative?<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>\nNo, the blast E value cannot be negative, as it represents a probability and probabilities are always positive.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"8_Is_the_blast_E_value_affected_by_the_size_of_the_database\"><\/span>8. Is the blast E value affected by the size of the database?<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>\nYes, the blast E value is influenced by the size of the database. Larger databases tend to have smaller E values due to the increased number of possible alignments.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"9_How_important_is_the_blast_E_value_in_sequence_analysis\"><\/span>9. How important is the blast E value in sequence analysis?<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>\nThe blast E value is a crucial parameter in sequence analysis as it provides a measure of the statistical significance of the alignment. It helps researchers in determining the reliability of their results.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"10_Why_is_a_small_E_value_desirable\"><\/span>10. Why is a small E value desirable?<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>\nA small E value indicates a low probability of obtaining the observed alignment by chance. It suggests a more reliable and significant match between the query and subject sequences.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"11_Can_the_blast_E_value_be_used_to_compare_different_queries\"><\/span>11. Can the blast E value be used to compare different queries?<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>\nYes, the blast E value can be used to compare the significance of alignments between different queries. A smaller E value would indicate a more significant alignment.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"12_Are_there_any_limitations_to_the_blast_E_value\"><\/span>12. Are there any limitations to the blast E value?<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>\nYes, the blast E value has some limitations. It assumes that the database is representative and that the query sequence is derived from a statistical population. Additionally, the E value does not provide information about the biological relevance of the alignment; it only measures statistical significance.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>What is a good blast E value? The E value is a numerical parameter that measures the significance of sequence alignments performed using the Basic Local Alignment Search Tool (BLAST). It represents the expected number of alignments occurring by chance when searching a database. A lower E value suggests a higher level of significance, indicating &#8230; <\/p>\n<p class=\"read-more-container\"><a title=\"What is a good blast E value?\" class=\"read-more button\" href=\"https:\/\/namso-gen.co\/blog\/what-is-a-good-blast-e-value\/#more-252413\">Read more<span class=\"screen-reader-text\">What is a good blast E value?<\/span><\/a><\/p>\n","protected":false},"author":64,"featured_media":107420,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[86279],"tags":[],"class_list":["post-252413","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-learn","no-featured-image-padding"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>What is a good blast E value?<\/title>\n<meta name=\"description\" content=\"What is a good blast E value? 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